Three different bacteria strains recently isolated (named 2 3 and 4 see the graphic) were analyzed Show more Three different bacteria strains recently isolated (named 2 3 and 4 see the graphic) were analyzed with two techniques used by molecular taxonomists (RFLP and full genome sequencing).
These techniques are successfully used in bacterial taxonomy. The goal of the assays was to identify the bacterial species using a reference bacterial strain identified with the number 1. The bacteria used as a reference was analyzed in parallel using the same methodology. The RFLP was performed using only one restriction enzyme and the fragments obtained were separated by electrophoresis and analyzed. The results obtained were clear and are shown in the Figure 1. It was demonstrated that the strains number 2 and 4 are different than 1. The strain number 3 showed the same pattern of bands as strain 1 after enzyme digestion; however after a full sequencing of its genomic information it was classified as a different species. Your task Explain in a few sentences why this is possible. In one complete sentence propose a simple modification of the RFLP technique to solve the problem without the need of full sequencing. Show less
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