YOU DECIDE TO CHANGE METHODS, AND THIS TIME YOU START BY MIXING 1 ML OF THE CULTURE OF BACTERIA INTO 9 MLS OF MEDIUM IN A TUBE (TUBE 1)

YOU DECIDE TO CHANGE METHODS, AND THIS TIME YOU START BY MIXING 1 ML OF THE CULTURE OF BACTERIA INTO 9 MLS OF MEDIUM IN A TUBE (TUBE 1)

You decide to change methods, and this time you start by mixing 1 mL of the culture of bacteria into 9 MLS of the medium in a tube (tube 1). You then repeat this process to carry out serial 1/10 dilutions, and transfer 0.1 mL from each tube to a plate and spread the inoculum over the surface. You then incubate the plates for 24 hours and count the resulting colonies on each plate. If you obtain 50 colonies on plate 2, prepared using the 2nd tube, how many bacteria were present in your original sample?