Assume that you work in a clinic and you must determine the leukocyte profile of patients. You have Show more Assume that you work in a clinic and you must determine the leukocyte profile of patients.
You have monoclonal antibodies directly conjugated to FITC (green) or APC (red) as shown in Table 1. You have every antibody in each color. Draw FACS quadrant diagrams (see previous diagrams) using the available reagents with white blood cell phenotypic data (in frequency) shown from two patients in Table 2. Label each axis of the FACS plots with the appropriate antibody and the fluorochrome used. Make two color data plots and answer the questions A-D on next page. Remember that the plots must add up to 100%. Table 1: Monoclonal antibodies available both in FITC and APC for each of the antibodies listed below. CD4 CD8 CD3 CD19 CD16 Table 2: Lymphocyte phenotype Patient A Patient B Total T Cells 60 20 CD4 40 10 CD8 20 10 B cells 30 78 NK cells 10 2 Compare CD4 vs. CD8 What is the normal ratio of CD4 to CD8? What are the ratios for Patient A and Patient B? Show less
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