. DNA from a strain of bacteria with genotype a+ b + c+ d+ e+ was isolated and used to transform a Show more Q1. DNA from a strain of bacteria with genotype a+ b + c+ d+ e+ was isolated and used to transform a strain of bacteria that was a- b- c- d- e-.
The transformed cells were tested for the presence of donated genes. The following genes were cotransformed: a+ and c+ b+ and a+ b+ and e+ d+ and e+. What is the order of genes a b c d e on the bacterial chromosome? . . . Q2. A donor strain of bacteria with genotype leu + gal pro + is infected with phages. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are leu gal + pro . The second strain is selected for leu + and the following cotransduction data are obtained: Donor Recipient Selected gene Cells with cotranduced gene (%) leu + gal pro + leu gal + pro leu + 26 pro + leu + 47 gal Which genes are closest leu and gal or leu and pro? Calculate distance between the genes. Show all possible maps . . Q3. The following E. coli Hfr strains donate their markers to F- cells in the order given: Hfr strain Order of gene transfer a 1 f e t a g Time (in min) 1 5 6 9 15 2 o c f h Time (in min) 1 2 17 20 3 u j o c r Time (in min) 4 13 22 23 32 4 n j u g t Time (in min) 7 13 22 31 40 Draw a map showing the relative order and distance of these genes in the circular chromosome of the original F+ stain from which all four Hfr stains were derived. Show less
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