EUKARYOTIC GENES CAN BE INTRODUCED INTO BACTERIA BY RECOMBINANT DNA TECHNIQUES. IF THE INTRODUCED GENE ENCODES A PROTEIN THAT IS ALSO FOUND IN BACTERIA—FOR EXAMPLE, A UNIVERSALLY USED GLYCOLYSIS ENZYME—THEN EXPRESSION OF THE EUKARYOTIC GENE MAY PRODUCE A PROTEIN THAT FUNCTIONS IN THE BACTERIAL CELL.
THE MOUSE GENE FOR A GLYCOLYSIS ENZYME IS INTRODUCED INTO AN E. COLI CELL THAT HAS A MUTANT GENE FOR THE BACTERIAL VERSION OF THE SAME ENZYME. EVEN THOUGH THE MOUSE ENZYME SHOULD FUNCTION IN THE BACTERIAL CELL AND RESTORE THE CELL’S ABILITY TO PERFORM GLYCOLYSIS, IT DOES NOT. PROVIDE A POSSIBLE REASON WHY THIS EXPERIMENT DOES NOT WORK AND PROPOSE A SOLUTION TO OVERCOME THE PROBLEM YOU SUGGEST.
Eukaryotic genes can be introduced into bacteria by recombinant DNA techniques. If the introduced gene encodes a protein that is also found in bacteria—for example, a universally used glycolysis enzyme—then expression of the eukaryotic gene may produce a protein that functions in the bacterial cell. The mouse gene for a glycolysis enzyme is introduced into an E. coli cell that has a mutant gene for the bacterial version of the same enzyme. Even though the mouse enzyme should function in the bacterial cell and restore the cell’s ability to perform glycolysis, it does not. Provide a possible reason why this experiment does not work and propose a solution to overcome the problem you suggest.
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