MANY SOIL BACTERIA HAVE GC RICH GENOMES.When cloning genes from these GC-rich bacteria, higher DNA melting temperatures and higher primer annealing temperatures must be used.

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Many soil bacteria have GC rich genomes. When cloning genes from these GC-rich bacteria, higher DNA melting temperatures and higher primer annealing temperatures must be used. Why do you think this would be the case compared to template DNA that is not as GC predominant?

MANY SOIL BACTERIA HAVE GC RICH GENOMES
March 20, 2018Orders In Progresscloning genes
Many soil bacteria have GC rich genomes. When cloning genes from these GC-rich bacteria, higher DNA melting temperatures and higher primer annealing temperatures must be used. Why do you think this would be the case compared to template DNA that is not as GC predominant?

MANY SOIL BACTERIA HAVE GC RICH GENOMES
March 20, 2018Orders In Progresscloning genes
Many soil bacteria have GC rich genomes. When cloning genes from these GC-rich bacteria, higher DNA melting temperatures and higher primer annealing temperatures must be used. Why do you think this would be the case compared to template DNA that is not as GC predominant?