The cells we are transforming lack LacZ in the bacterial genome. What do you expect would happen if Show more The cells we are transforming lack LacZ in the bacterial genome.
What do you expect would happen if we transformed LacZ+ cells with your plasmid? Why would it be a problem? In this experiment each plasmid will contain the following genes. 1) A gene AmpR that encodes a gene that confers antibiotic resistance 2) A gene LacZ that codes for beta-galactosidase which is an enzyme that can break down lactose into its constituent monosaccharrides glucose and galactose 3) An origin of replication so that the plasmid can be replicated. 4) A multiple cloning site with a number of sites recognized by a variety of restriction enzymes. Show less
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