The following sequence shows a bacterial gene starting +1 (transcription initiation site). Promoter Show more The following sequence shows a bacterial gene starting +1 (transcription initiation site). Promoter sequence is not shown.
The top strand is in 5 to 3 direction and the bottom strand is in 3 to 5 direction. ATTGTGAGCGGATAACAATGTCACACAGGAAACAGCTAAGACCATGTTT -+-+de+f-c++ TAACACTCGCCTATTGTTACAGTGTGTCCTTTGTCGATTCTGGTACAAA i). Write the sequence of mRNA produced from this DNA. ii) What are the first six amino acids of the resulting protein? Use genetic code table from your book. iii) Does translation terminate at the underlined TAA at position (c bold)? Why or why not? iv) How would your answer to ii) if you replace the nucleotide C (indicated with bold d) with a G. v) What would be the first six amino acids if you deleted the nucleotide C (indicated with bold d)? Also would the underlined TAA at position (c bold) result in termination of polypeptide synthesis. What kind of mutation would this represent? vi) How would your answer to if you change nucleotide A (indicated with f bold) with a G. What kind of mutation would this represent? Show less
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