YOU HAVE DISCOVERED A GENE THAT IS DOWN-REGULATED 10-FOLD IN BREAST CANCER. YOUR COMMITTEE ASKS YOU THE FOLLOWING: 1. IS THE GENE EPIGENETICALLY DOWN-REGULATED?
IF YOU ARE NOT SURE, DESIGN EXPERIMENTS TO VERIFY THIS. 2. IF YOU DO FIND IT TO BE EPIGENETICALLY SUPPRESSED, DESCRIBE 3 MOLECULAR MECHANISMS THAT COULD EXPLAIN THIS 3. DESIGN EXPERIMENTS TO DISTINGUISH BETWEEN THE 3 MECHANISMS YOU DESCRIBE ABOVE IF IT IS NOT EPIGENETICALLY REGULATED, WHAT COULD ACCOUNT FOR THE DECREASED EXPRESSION IN BREAST CANCER?
You have discovered a gene that is down-regulated 10-fold in breast cancer. Your committee asks you the following: 1. Is the gene epigenetically down-regulated? If you are not sure, design experiments to verify this. 2. If you do find it to be epigenetically suppressed, describe 3 molecular mechanisms that could explain this 3. Design experiments to distinguish between the 3 mechanisms you describe above If it is not epigenetically regulated, what could account for the decreased expression in breast cancer?
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