Question Penicillase is an enzyme secreted by bacteria to inactivate the antibiotic penicillin. The enzyme has a molar mass of 30000 and a single active site.
The catalytic rate constant is 2000 s-1 and the Michaelis constant is KM = 5 x 10-5 M. In response to treatment with 5 microM of penicillin a 1 mL (milliliter) suspension of bacterial cells secretes 0.04 micrograms of penicillase. A. Assuming that the enzyme quickly equilibrates with its substrate what fraction of the enzyme will be complexed with penicillin in the early stages of the reaction? Fraction complexed B. How long will it take for half of the penicillin to become inactivated? seconds C. What concentration of penicillin would cause the substrate to react at half its maximum velocity? molar
Show transcribed image text Penicillase is an enzyme secreted by bacteria to inactivate the antibiotic penicillin. The enzyme has a molar mass of 30000 and a single active site. The catalytic rate constant is 2000 s-1 and the Michaelis constant is KM = 5 times 10-5 M. In response to treatment with 5 microM of penicillin a 1 mL (milliliter) suspension of bacterial cells secretes 0.04 micrograms of penicillase. Assuming that the enzyme quickly equilibrates with its substrate what fraction of the enzyme will be complexed with penicillin in the early stages of the reaction?
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