Hfr bacteria with the genotype lac+met+his+trp+thr+strS are mixed with F- bacteria with the genotype Show more Hfr bacteria with the genotype lac+met+his+trp+thr+strS are mixed with F- bacteria with the genotype lac-met-his-trp-thr-strR.
Mating is interrupted at 20 minutes and replica plating is used to determine the percentage of exconjugants that have the following phenotypes. Gene and percentage Of Colonies: lac + 100% trp+ 78% thr+ 85% his+ 67% met+ 54% a. what type of media should be used to select for exconjugants (and ensure that none of the original Hfr and F- bacteria will grow)? b. draw a map of the bacterial chromosome from the Hfr strain used in this interrupted mating experiment. Include the F plasmid integration site as well as the direction and order of gene transfer. Show less
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